LRP6 antibody (pSer1490) (HRP)

Catalog No. ABIN743567

The Rabbit Polyclonal anti-LRP6 antibody is suitable to detect LRP6 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC (p) and IHC (fro).

Quick Overview for LRP6 antibody (pSer1490) (HRP) (ABIN743567)

Target: LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LRP6 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-LRP6 Antibody (HRP)

Binding Specificity: pSer1490

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human LRP6 around the phosphorylation site of Ser1490

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for LRP6

Target: LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))

Alternative Name: LRP6

Background:

Synonyms: ADCAD2, Low-density lipoprotein receptor-related protein 6, LRP-6, LRP6

Background: Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation (By similarity).

Gene ID: 4040

UniProt: O75581

Pathways: WNT Signaling, Tube Formation