ERAS antibody (AA 13-42)

Catalog No. ABIN7870475

This Rabbit Polyclonal antibody specifically detects ERAS in WB, IF, IHC (p) and FACS. It exhibits reactivity toward Human.

Quick Overview for ERAS antibody (AA 13-42) (ABIN7870475)

Target: ERAS (ES cell expressed Ras (ERAS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERAS antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-ERAS Antibody

Binding Specificity: AA 13-42

Purpose: ERAS Antibody / GTPase ERas

Purification: Purified

Immunogen: A portion of amino acids 13-42 from the human protein was used as the immunogen for the ERAS antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the ERAS antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the ERAS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for ERAS

Target: ERAS (ES cell expressed Ras (ERAS))

Alternative Name: ERAS

Background: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells.

UniProt: Q7Z444