PCYT2 antibody (AA 32-389)

Catalog No. ABIN7873920

The Rabbit Polyclonal anti-PCYT2 antibody is suitable to detect PCYT2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, ICC, FACS, IF and IP.

Quick Overview for PCYT2 antibody (AA 32-389) (ABIN7873920)

Target: PCYT2 (Phosphate Cytidylyltransferase 2, Ethanolamine (PCYT2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCYT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-PCYT2 Antibody

Binding Specificity: AA 32-389

Purpose: PCYT2 Antibody / Ethanolamine-phosphate cytidylyltransferase

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PCYT2 recombinant protein (Position: D32-F389) was used as the immunogen for the PCYT2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PCYT2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PCYT2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PCYT2

Target: PCYT2 (Phosphate Cytidylyltransferase 2, Ethanolamine (PCYT2))

Alternative Name: PCYT2

Background: PCYT2 antibody detects Ethanolamine-phosphate cytidylyltransferase, a key enzyme in the CDP-ethanolamine branch of the Kennedy pathway for phosphatidylethanolamine synthesis. PCYT2 catalyzes the formation of CDP-ethanolamine from CTP and phosphoethanolamine, an essential step in the biosynthesis of membrane phospholipids. The PCYT2 antibody is used in lipid metabolism, neurobiology, and mitochondrial research to study phospholipid homeostasis and membrane biogenesis.

PCYT2 is encoded by the PCYT2 gene located on human chromosome 17q25.3. The protein is approximately 43 kilodaltons and localized predominantly in the endoplasmic reticulum. It functions as a rate-limiting enzyme that determines phosphatidylethanolamine synthesis levels, thereby influencing membrane fluidity, mitochondrial morphology, and cellular signaling. PCYT2 activity is critical for maintaining the structural integrity of cell membranes and organelles, particularly in the nervous system and muscle tissue.

Using the PCYT2 antibody, researchers can identify the 43 kilodalton protein by western blot and immunofluorescence, where it shows perinuclear localization corresponding to the ER network. Genetic and biochemical studies have shown that PCYT2 deficiency results in reduced phosphatidylethanolamine production, leading to mitochondrial dysfunction, abnormal lipid droplet accumulation, and neurodevelopmental impairment. Mutations in PCYT2 cause hereditary spastic paraplegia type 82 and neuromuscular lipid metabolism disorders.

PCYT2 expression is regulated by nutritional and hormonal cues, including insulin and fatty acid levels. It interacts with lipid transfer proteins and participates in the coordination between the Kennedy pathway and mitochondrial phospholipid synthesis. NSJ Bioreagents provides a validated PCYT2 antibody supporting research into membrane biosynthesis, lipid metabolism disorders, and mitochondrial integrity.

UniProt: Q99447