STK39 antibody (AA 346-376)

Catalog No. ABIN7874119

The Rabbit Polyclonal anti-STK39 antibody (ABIN7874119) specifically detects STK39 in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for STK39 antibody (AA 346-376) (ABIN7874119)

Target: STK39 (serine threonine Kinase 39 (STK39))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STK39 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-STK39 Antibody

Binding Specificity: AA 346-376

Purpose: SPAK Antibody / STK39

Purification: SAS precipitation

Immunogen: A portion of amino acids 346-376 from the human protein were used as the immunogen for the SPAK antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the SPAK antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the SPAK antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for STK39

Target: STK39 (serine threonine Kinase 39 (STK39))

Alternative Name: SPAK

Background: SPAK is a serine/threonine kinase containing an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain, a nuclear localization signal, a consensus caspase cleavage recognition motif, and a C-terminal region. Northern blot analysis detects ubiquitous expression, most abundantly in brain and pancreas. SPAK can phosphorylate itself and an exogenous substrate in vitro. SPAK immunoprecipitates from transfected mammalian cells in a complex with another serine/threonine kinase that phosphorylates catalytically inactive SPAK. SPAK activates the p38 MAP kinase pathway in cotransfection assays. Full-length SPAK is expressed in the cytoplasm in transfected cells, while a mutant corresponding to caspase-cleaved STK39 localizes predominantly in the nucleus.

UniProt: Q9UEW8