OAS2 antibody (AA 35-742)

Catalog No. ABIN7874178

The Rabbit Polyclonal anti-OAS2 antibody is suitable to detect OAS2 in samples from Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for OAS2 antibody (AA 35-742) (ABIN7874178)

Target: OAS2 (2'-5'-Oligoadenylate Synthetase 2, 69/71kDa (OAS2))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OAS2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-OAS2 Antibody

Binding Specificity: AA 35-742

Purpose: Oas2 Antibody / 2-5-Oligoadenylate synthetase 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived mouse Oas2 recombinant protein (Position: H35-K742) was used as the immunogen for the Oas2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Oas2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Oas2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for OAS2

Target: OAS2 (2'-5'-Oligoadenylate Synthetase 2, 69/71kDa (OAS2))

Alternative Name: Oas2

Background: OAS2 antibody detects 2'-5'-oligoadenylate synthetase 2, a double-stranded RNA-activated enzyme encoded by the OAS2 gene located on chromosome 12q24.13. OAS2 plays a central role in the innate immune response to viral infection by synthesizing 2'-5'-linked oligoadenylates (2-5A) upon activation by viral double-stranded RNA. These 2-5A molecules subsequently activate RNase L, leading to degradation of viral and cellular RNA and inhibition of viral replication. OAS2 is expressed in most tissues but is strongly upregulated in response to interferon signaling, particularly in immune cells such as macrophages, dendritic cells, and lymphocytes.

Structurally, OAS2 is a 113 kDa protein containing two functional OAS catalytic domains, allowing higher polymerization activity than other family members such as OAS1. It belongs to the 2'-5'-oligoadenylate synthetase family of nucleotidyltransferases, characterized by a conserved polymerase fold and dsRNA-binding motifs. OAS2 localizes to the cytoplasm, often associating with ribosomes and endoplasmic reticulum membranes where viral replication typically occurs.

Functionally, OAS2 acts as a critical antiviral effector enzyme in interferon-stimulated pathways. Upon recognition of viral RNA, OAS2 catalyzes ATP polymerization into 2-5A molecules that bind and activate latent RNase L. This leads to RNA cleavage, apoptosis of infected cells, and amplification of antiviral signaling. OAS2 also interacts with pattern recognition receptors such as MDA5 and RIG-I, coordinating interferon response cascades. In addition to antiviral defense, OAS2 influences immune regulation, apoptosis, and inflammatory cytokine expression.

OAS2 expression is strongly induced by type I and type III interferons. Genetic variants in OAS2 have been associated with altered susceptibility to viral infections including SARS-CoV-2, hepatitis C, and West Nile virus. Dysregulation of OAS2 can contribute to autoimmune disorders through excessive RNA degradation. Pathway associations include interferon signaling, RNA decay, and innate immune activation. During development, OAS2 expression in immune progenitors supports early antiviral competence.

The OAS2 antibody from NSJ Bioreagents is a valuable reagent for studying antiviral mechanisms, interferon signaling, and immune regulation.

UniProt: E9Q9A9

Pathways: Hepatitis C