NARF antibody (AA 41-353)

Catalog No. ABIN7874835

The Rabbit Polyclonal anti-NARF antibody is suitable to detect NARF in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for NARF antibody (AA 41-353) (ABIN7874835)

Target: NARF (Nuclear Prelamin A Recognition Factor (NARF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NARF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-NARF Antibody

Binding Specificity: AA 41-353

Purpose: NARF Antibody / Nuclear prelamin A recognition factor

Purification: Antigen affinity purified

Immunogen: E. coli-derived recombinant human protein (amino acids H41-Q353) was used as the immunogen for the NARF antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NARF antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NARF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NARF

Target: NARF (Nuclear Prelamin A Recognition Factor (NARF))

Alternative Name: NARF

Background: Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.

UniProt: Q9UHQ1