PMM2 antibody (AA 48-246)

Catalog No. ABIN7875183

The Rabbit Polyclonal anti-PMM2 antibody is suitable to detect PMM2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF, ICC and FACS.

Quick Overview for PMM2 antibody (AA 48-246) (ABIN7875183)

Target: PMM2 (Phosphomannomutase 2 (PMM2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PMM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-PMM2 Antibody

Binding Specificity: AA 48-246

Purpose: PMM2 Antibody / Phosphomannomutase 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PMM2 recombinant protein (Position: D48-S246) was used as the immunogen for the PMM2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PMM2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PMM2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PMM2

Target: PMM2 (Phosphomannomutase 2 (PMM2))

Alternative Name: PMM2

Background: PMM2 antibody detects Phosphomannomutase 2, encoded by the PMM2 gene on chromosome 16p13.2. PMM2 antibody is widely used in research on glycosylation disorders, metabolism, and enzymology. PMM2 is a cytoplasmic enzyme that catalyzes the interconversion of mannose-6-phosphate and mannose-1-phosphate. This reaction is a key step in the synthesis of GDP-mannose, which is essential for N-linked glycosylation, glycosylphosphatidylinositol anchor formation, and protein glycan modification.

Structurally, PMM2 is a ~28 kDa enzyme belonging to the phosphohexomutase family. It forms dimers and requires magnesium ions for catalytic activity. The enzyme adopts an alpha/beta fold typical of sugar phosphate mutases and contains an active-site serine residue that is phosphorylated during catalysis. PMM2 is highly conserved among species, underscoring its fundamental role in cell biology.

Functionally, PMM2 is critical for proper glycoprotein biosynthesis. Its deficiency results in impaired glycosylation, disrupting protein folding, trafficking, and function. This enzyme is ubiquitously expressed, with highest activity in tissues requiring extensive glycosylation, such as liver and brain. Researchers use PMM2 antibody to study glycosylation pathways, metabolic regulation, and congenital disorders of glycosylation (CDG).

Clinically, mutations in PMM2 cause congenital disorder of glycosylation type Ia (CDG-Ia), also known as Jaeken syndrome. This is the most common CDG and is characterized by developmental delay, hypotonia, coagulopathy, and multi-organ dysfunction. More than 100 pathogenic variants of PMM2 have been described, with variable severity. Therapeutic approaches under investigation include dietary supplementation and enzyme replacement strategies. NSJ Bioreagents provides PMM2 antibody for research in glycosylation disorders, enzymology, and disease models.

Experimentally, PMM2 antibody is applied in western blotting to detect the ~28 kDa protein, in immunohistochemistry to study tissue distribution, and in immunofluorescence to assess subcellular localization. Enzymatic assays combined with PMM2 antibody provide functional insights into sugar phosphate metabolism.

UniProt: O15305