PPP1R12A antibody (Myosin Phosphatase, Target Subunit 1) (AA 690-701)

Details for Product anti-PPP1R12A Antibody No. ABIN744068
Antigen
Epitope
AA 690-701, pThr696
33
25
15
12
9
8
8
8
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
174
137
116
10
9
9
5
5
3
3
3
3
2
1
Host
Rabbit
172
1
1
Clonality
Polyclonal
Conjugate
This PPP1R12A antibody is un-conjugated
9
9
9
9
9
9
9
2
2
2
2
2
2
2
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
106
53
52
27
20
9
8
2
2
2
1
1
Options
Immunogen KLH conjugated synthetic phosphopeptide derived from human around the phosphorylation site of Ser696
Isotype IgG
Specificity This phosphorylation site is homologous to that of Thr694 in Mouse and Thr697 in Rat. The region containing this phosphorylation site is known to be homologous to that of the related protein, PP12C, and will react with this protein when phosphorylated at Thr560 in Human and Mouse, and Thr561 in Rat.
Purification Purified by Protein A.
Antigen
Alternative Name Mypt1 (PPP1R12A Antibody Abstract)
Background

Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.

Subcellular location: Cytoplasm, Helical

Synonyms: MBS, M130, MYPT1, Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, Protein phosphatase myosin-binding subunit, PPP1R12A

Gene ID 4659
UniProt O14974
Pathways M Phase
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
Image no. 1 for anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 690-701), (pThr696) antibody (ABIN744068) 293T lysates probed with MYPT1 (Thr696) Polyclonal Antibody, Unconjugated at 1:300 d...
Image no. 2 for anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 690-701), (pThr696) antibody (ABIN744068) Rat BRL-3A lysates probed with p-MYPT1(Thr696) Polyclonal Antibody, Unconjugated at ...
Image no. 3 for anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 690-701), (pThr696) antibody (ABIN744068) Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-Phospho-MYPT1(Th...