Mre11 antibody (pSer676) (HRP)

Catalog No. ABIN744167

The Rabbit Polyclonal anti-Mre11 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect Mre11 in samples from Human.

Quick Overview for Mre11 antibody (pSer676) (HRP) (ABIN744167)

Target: Mre11 (MRE11A) (MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Mre11 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Mre11 Antibody (HRP)

Binding Specificity: pSer676

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from rat Mre11 around the phosphorylation site of Ser676

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Mre11

Target: Mre11 (MRE11A) (MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))

Alternative Name: Mre11

Background:

Synonyms: Mre 11, MRE 11a, MRE 11b, MRE11 homolog 1, MRE11 meiotic recombination 11 homolog A, MRE11a, MRE11b, AT like disease, Ataxia telangiectasia disorder like, Ataxia-telangiectasia disorder-like, ATLD, DNA recombination and repair protein, Double strand break repair protein MRE11A, Double-strand break repair protein MRE11A, endo/exonuclease Mre11. HNGS1, meiotic recombination S. cerevisiae 11 homolog A, meiotic recombination 11 homolog A S. cerevisiae, meiotic recombination 11 homolog A, M mMRE11A.MRE11_RAT

Background: Mre11 is a component of the MRN complex, which plays a critical role in double-strand break repair (DSB), DNA recombination, maintenance of telomere integrity, and meiosis. Mre11 has both single-strand endonuclease and double strand-specific 3'-5' exonuclease activities. Rad50, another component of the MRN complex, is postulated to bind to DNA ends and hold them in close proximity, thus facilitating searches for regions of sequence homology, and also possibly stimulating the activity of DNA ligases and/or restricting Mre11 activity. Mutations in Mre11 may cause breast cancer.

Gene ID: 4361

Pathways: DNA Damage Repair