Tumor Suppressor ARF (CDKN2A) (AA 72-101) antibody

Catalog No. ABIN7876617

The Rabbit Polyclonal anti-Tumor Suppressor ARF antibody is suitable to detect Tumor Suppressor ARF in samples from Human. It has been validated for IHC (p), FACS and WB.

Quick Overview for Tumor Suppressor ARF (CDKN2A) (AA 72-101) antibody (ABIN7876617)

Target: Tumor Suppressor ARF (CDKN2A)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Western Blotting (WB)

Product Details

Binding Specificity: AA 72-101

Purpose: Tumor suppressor ARF Antibody / CDKN2A / MLM

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 72-101 from the human protein was used as the immunogen for the Tumor suppressor ARF antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Tumor suppressor ARF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Tumor suppressor ARF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details

Target: Tumor Suppressor ARF (CDKN2A)

Alternative Name: Tumor suppressor ARF

Background: This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene, this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, MDM1, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.

UniProt: Q8N726