NUMA1 antibody (pSer395) (HRP)

Catalog No. ABIN744452

The Rabbit Polyclonal anti-NUMA1 antibody has been validated for ELISA, IHC (p) and IHC (fro). It is suitable to detect NUMA1 in samples from Human.

Quick Overview for NUMA1 antibody (pSer395) (HRP) (ABIN744452)

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUMA1 antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NUMA1 Antibody (HRP)

Binding Specificity: pSer395

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human NuMA around the phosphorylation site of Ser395

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NUMA1

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Alternative Name: NuMA

Background:

Synonyms: NUMA 1, NuMA protein, NUMA1, SP H antigen, Structural nuclear protein, Centrophilin stabilizes mitotic spindle in mitotic cells, Nuclear mitotic apparatus protein 1, Nuclear mitotic apparatus protein.

Background: NuMA (Nuclear Mitotic Apparatus Protein) is an intranuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200 kDa during apoptosis.

Gene ID: 4926

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase