CNR2 antibody (3rd Intracellular Loop)

Catalog No. ABIN7884843

This Rabbit Polyclonal antibody specifically detects CNR2 in WB and IHC. It exhibits reactivity toward Rat and Mouse.

Quick Overview for CNR2 antibody (3rd Intracellular Loop) (ABIN7884843)

Target: CNR2 (Cannabinoid Receptor 2 (CNR2))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-CNR2 Antibody

Binding Specificity: 3rd Intracellular Loop, AA 228-242

Purpose: A Rabbit Polyclonal Antibody to Cannabinoid Receptor 2

Predicted Reactivity: Mouse - identical Human - 14 out of 15 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DRQVPGIARMRLDVR, corresponding to amino acid residues 228-242 of rat CB2 receptor

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100-1:400

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235032)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CNR2

Target: CNR2 (Cannabinoid Receptor 2 (CNR2))

Alternative Name: CB2

Background:

Synonyms: CB2, CNR2

Description: Cannabinoids have been used as pain relievers in Eastern medicine for many years.1 To date, two specific cannabinoid receptors have been identified: cannabinoid receptor 1 (CB1) and cannabinoid receptor 2 (CB2).2 The cannabinoid receptors can be distinguished by their amino acid sequence, signaling mechanisms and tissue distribution.2 Both receptors belong to the G-protein coupled receptor superfamily and are coupled to Gi/0 G protein.2,3The CB2 receptor is highly expressed in cells of the immune system such as macrophages, lymphocytes natural killer cells and mast cells but has also been shown to be expressed, by both, in situ-hybridization and in immunohistochemistry, in spleen, thymus, and pancreas.1,2,4 CB2 expression in the brain is still much less characterized than that of CB1. Recently, it was demonstrated that CB2 is expressed in the brain and might have a role in controlling fundamental processes such as proliferation and survival of neural cells.5,6Overexpression of CB2 was reported in several cancers such as prostate, glioma and acute myeloid leukemias.6 In human astrocytoma a direct relationship between CB2 expression and tumor malignancy was demonstrated. Activation of CB2 in vivo by its agonist JWH-133, completely blocked cell growth. In C6 glioma, it was shown that activation of the CB2 by JWH-133 resulted in the internalization of only the CB2 and not CB1 leading to apoptosis of the cells. This may well be a new approach for the treatment of glioma.7

Gene ID: 57302

UniProt: Q9QZN9