Complexin 1 antibody (Intracellular, N-Term)

Catalog No. ABIN7884894

This Rabbit Polyclonal antibody specifically detects Complexin 1 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Complexin 1 antibody (Intracellular, N-Term) (ABIN7884894)

Target: Complexin 1 (CPLX1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Complexin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-Complexin 1 Antibody

Binding Specificity: AA 14-27, Intracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Complexin-1 and Complexin-2

Predicted Reactivity: Complexin 1: Mouse,human - identicalComplexin 2: Rat,mouse,human - 13,14 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KDMGKMLGGDEEKD, corresponding to amino acid residues 14-27 of rat Complexin-1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Complexin 1

Target: Complexin 1 (CPLX1)

Alternative Name: Complexin 1

Background:

Synonyms: Complexin 1, Complexin I, Synaphin-2, Complexin 2, Complexin II, Synaphin-1

Description: Complexins (1 through 4) are small cytosolic proteins that bind tightly to a complex of SNARE proteins to regulate synaptic vesicle membrane fusion via its interaction with synaptotagmin. Complexins activate the quick release of neurotransmitters, triggered by calcium release. They also regulate the "spontaneous release" of neurotransmitters1-3.Complexins are evolutionarily conserved in mammals, Drosophila, and Caenorhabditis elegans1,2. They are divided into four structural domains: flexible N- and C-terminal domains, an accessory domain, and a central α-helical domain. The N-terminal domain plays a role in activation of fast synchronous release in murine neurons and in isolated chromaffin cells. The C-terminal domain binds to phospholipids and is important for regulating spontaneous release in neuronal cultures and suppressing Ca2+-independent fusion in vitro. The accessory domain is necessary for regulating spontaneous release and the central domain is required for all functions1,2.

Gene ID: 64833, 116657

UniProt: P63041, P84087

Pathways: Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport