Mucolipin 2 (MCOLN2) (AA 212-228), (Intracellular), (N-Term) antibody

Catalog No. ABIN7885149

This Rabbit Polyclonal antibody specifically detects Mucolipin 2 in WB, IC, IF and IHC. It exhibits reactivity toward Mouse, Human and Rat.

Quick Overview for Mucolipin 2 (MCOLN2) (AA 212-228), (Intracellular), (N-Term) antibody (ABIN7885149)

Target: Mucolipin 2 (MCOLN2)

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunochromatography (IC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Grade: Carrier-free, KO Validated

Product Details

Binding Specificity: AA 212-228, Intracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to TRPML2 (Mucolipin-2) Channel

Predicted Reactivity: human - 13,Rat - 16,17 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RLDFYRLVQVDISFALK, corresponding to amino acid residues 212-228 of mouse TRPML2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: 1:50

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235975)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details

Target: Mucolipin 2 (MCOLN2)

Alternative Name: Mcoln2

Background:

Synonyms: Mcoln2

Description: The endolysosome system takes part in important cellular functions such as membrane trafficking, protein transport, autophagy and signal transduction1. Endosomes result from endocytosis of the plasma membrane and lysosomes (which are derived from late endosomes) conatin mainly hydrolytic enzymes and generally have a low internal pH 1. Like the endoplasmic reticulum (ER), endolysosomes also store Ca2+ (luminal Ca2+ concentration: 0.5 mM)1,2, and similarly to Ca2+ release from the ER, Ca2+ from endolysosomes may also play an important role in various signaling events. To date such candidates include members of the TRP super-family of ion channels and the two-pore Ca2+ channels (TPCs)1,3,4.TRPMLs, also termed mucolipins, are members of the TRP channels. In mammals, three TRPMLs are known to date (TRPML1-3 or MCOLN1-3). They are all localized to endolysosomes, although when over expressed in heterologous systems, TRPML3 is found on the plasma membrane1,5. These channels are Ca2+ permeable and display inward rectifying current properties1,5. Like all members of this family, TRPMLs have six transmembrane domains and intracellular N- and C-termini (relatively short tails compared to other members). They are characterized by an exceptionally large extracellular (luminal) loop between transmembrane domains 1 and 2, and N-glycosylation sites are present in the first extracellular (luminal) loop5.In mammals, TRPML1 is expressed in a ubiquitous manner and shows highest expression in the brain, kidney, spleen, liver and heart1,6. TRPML2 and TRPML3 are less widely expressed. Interestingly, in mouse, two splice variants exist for TRPML2. The shorter variant is more broadly expressed and is dominant over the longer variant in the thymus, spleen and kidney1,7. TRPML3 is highly detected in the thymus, lung, kidney, spleen and eye1,7,8, some epithelial cells1,9 and brain10.Pathologies related to these channels include type IV mucolipidosis, a neurodegenetative disease characterized by retardation and retinal degeneration caused by a loss of function mutation in the gene encoding TRPML1. In contrast, a gain of function mutation in TRPML3, in mice, causes deafness, and pigmentation defects11.

Gene ID: 68279

UniProt: Q8K595