SRF antibody (pSer103)

Catalog No. ABIN745898

The Rabbit Polyclonal anti-SRF antibody has been validated for WB, ELISA, IF (p), IF (cc), IHC (p) and IHC (fro). It is suitable to detect SRF in samples from Mouse. There is 1 publication available.

Quick Overview for SRF antibody (pSer103) (ABIN745898)

Target: SRF (Serum Response Factor (SRF))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SRF Antibody

Binding Specificity: pSer103

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human SRF around the phosphorylation site of Ser103

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SRF antibody (ABIN745898)

Stern, Haverkamp, Sinske, Tedeschi, Naumann, Di Giovanni, Kochanek, Nordheim, Knöll: "The transcription factor serum response factor stimulates axon regeneration through cytoplasmic localization and cofilin interaction." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 48, pp. 18836-48, (2013) (PubMed).

Target Details for SRF

Target: SRF (Serum Response Factor (SRF))

Alternative Name: SRF

Background:

Synonyms: Serum Response Factor SRF phospho S103, SRF phospho Ser103, P-SRF Ser103, c fos serum response element binding factor, Serum Response Factor SRF, c fos serum response element binding transcription factor, ELK3, ERP, MCM 1, MCM-1, MCM1, OTTHUMP00000039820, SAP2, Serum response factor, SRF, SRF serum response factor c fos serum response element binding transcription factor, SRF_HUMAN.

Background: This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways, for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). [provided by RefSeq, Jul 2008].

Gene ID: 6722