FBXO32 antibody (AA 31-130)

Catalog No. ABIN747698

The Rabbit Polyclonal anti-FBXO32 antibody has been validated for WB, ELISA, IF (cc), FACS, IF (p), IHC (p) and IHC (fro). It is suitable to detect FBXO32 in samples from Human and Rat. There is 1 publication available.

Quick Overview for FBXO32 antibody (AA 31-130) (ABIN747698)

Target: FBXO32 (F-Box Protein 32 (FBXO32))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO32 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-FBXO32 Antibody

Binding Specificity: AA 31-130

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Fbx32

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-FBXO32 antibody (ABIN747698)

Lin, Hanson, Betik, Brennan-Speranza, Hayes, Levinger: "Hindlimb Immobilization, But Not Castration, Induces Reduction of Undercarboxylated Osteocalcin Associated With Muscle Atrophy in Rats." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 31, Issue 11, pp. 1967-1978, (2016) (PubMed).

Target Details for FBXO32

Target: FBXO32 (F-Box Protein 32 (FBXO32))

Alternative Name: Fbx32

Background:

Synonyms: Fbx32, MAFbx, F-box only protein 32, Atrogin-1, Muscle atrophy F-box protein, FBXO32

Background: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.

Gene ID: 114907

UniProt: Q969P5