FBXO32 antibody (F-Box Protein 32) (Internal Region)

Details for Product anti-FBXO32 Antibody No. ABIN570946
Antigen
  • MGC56479
  • zgc:56479
  • Fbx32
  • MAFbx
  • 4833442G10Rik
  • AI430017
  • ATROGIN1
  • Gm20361
  • Atrogin1
  • F-box protein 32
  • fbxo32
  • FBXO32
  • Fbxo32
Epitope
Internal Region
6
3
2
2
2
2
2
2
1
1
Reactivity
Mouse (Murine)
88
57
54
2
2
2
2
2
2
1
Host
Goat
80
9
6
Clonality
Polyclonal
Conjugate
This FBXO32 antibody is un-conjugated
6
4
4
4
4
4
4
3
2
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
64
23
21
13
5
5
4
3
2
2
1
1
Options
Purpose FBXO32
Immunogen Peptide with sequence C-NSKTKTQYFHQEK, from the internal region of the protein sequence according to NP_478136.1.
Blocking Peptide Blocking peptide for this product available: ABIN2815661
Sequence NSKTKTQYFH QEK
Isotype IgG
Specificity This antibody is expected to recognize reported isoform 1 (NP_478136.1).
Predicted Reactivity Human, Mouse, Rat, Cow, Pig
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Plasmids, Primers & others Plasmids, Primers & others FBXO32 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name FBXO32 (FBXO32 Antibody Abstract)
Background FBXO32, F-box protein 32, FLJ32424, Fbx32, MAFbx, MGC33610, F-box only protein 32, atrogin 1, muscle atrophy F-box protein
Gene ID 114907
NCBI Accession NP_478136
Application Notes

DS WB Results: Approx. 40 kDa band observed in Mouse Skeletal Muscle lysates (calculated MW of 41.5 kDa according to Mouse NP_080622.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat and Pig Skeletal Muscle, and Pig Heart for this particular batch.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Supplier Images
Image no. 1 for anti-F-Box Protein 32 (FBXO32) (Internal Region) antibody (ABIN570946) ABIN570946 (0.3µg/ml) staining of Mouse Skeletal Muscle lysate (35µg protein in RIPA ...