SPHK2 antibody (AA 101-200)

Catalog No. ABIN748613

The Rabbit Polyclonal anti-SPHK2 antibody has been validated for ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect SPHK2 in samples from Human, Mouse and Rat. There are 4+ publications available.

Quick Overview for SPHK2 antibody (AA 101-200) (ABIN748613)

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SPHK2 Antibody

Binding Specificity: AA 101-200

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SPHK2

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SPHK2 antibody (ABIN748613)

Tran, Jersmann, Truong, Hamon, Roscioli, Ween, Pitman, Pitson, Hodge, Reynolds, Hodge: "Disrupted epithelial/macrophage crosstalk via Spinster homologue 2-mediated S1P signaling may drive defective macrophage phagocytic function in COPD." in: PLoS ONE, Vol. 12, Issue 11, pp. e0179577, (2017) (PubMed).

Zhang, Li, Yuan, Zhao: "Hypoxic preconditioning protects cardiomyocytes against hypoxia/reoxygenation-induced cell apoptosis via sphingosine kinase 2 and FAK/AKT pathway." in: Experimental and molecular pathology, Vol. 100, Issue 1, pp. 51-8, (2016) (PubMed).

Tani, Tabuchi, Hara: "Hair Cell Loss Induced by Sphingosine and a Sphingosine Kinase Inhibitor in the Rat Cochlea." in: Neurotoxicity research, Vol. 29, Issue 1, pp. 35-46, (2016) (PubMed).

Tran, Barnawi, Ween, Hamon, Roscioli, Hodge, Reynolds, Pitson, Davies, Haberberger, Hodge: "Cigarette smoke inhibits efferocytosis via deregulation of sphingosine kinase signaling: reversal with exogenous S1P and the S1P analogue FTY720." in: Journal of leukocyte biology, Vol. 100, Issue 1, pp. 195-202, (2016) (PubMed).

Target Details for SPHK2

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Alternative Name: SPHK2

Background:

Synonyms: SK 2, SK-2, SPK 2, SPK-2, Sphingosine kinase 2, SPHK2

Background: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.

Gene ID: 56848

UniProt: Q9NRA0

Pathways: VEGF Signaling