STRADA antibody (AA 1-100) (HRP)

Catalog No. ABIN751597

This Rabbit Polyclonal antibody specifically detects STRADA in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for STRADA antibody (AA 1-100) (HRP) (ABIN751597)

Target: STRADA (STE20-Related Kinase Adaptor alpha (STRADA))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STRADA antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-STRADA Antibody (HRP)

Binding Specificity: AA 1-100

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human LYK5

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for STRADA

Target: STRADA (STE20-Related Kinase Adaptor alpha (STRADA))

Alternative Name: LYK5

Background:

Synonyms: Serologically defined breast cancer antigen NY BR 96, STRAD alpha, Protein kinase LYK5, STE20 related adapter protein, STRAD, STRAA_HUMAN.

Background: Members of the STE-20 like kinase family are known to stimulate MAPK pathways by directly activating MAPKKK. LYK5 is a novel pseudokinase member of this family consisting of a STE-20 like kinase domain but lacks several residues that are required for its catalytic activity. It specifically binds LKB1 and plays a key role in regulating the tumor suppressor activities of LKB1. It functions as an upstream activator of LKB1 and also directs the sub-cellular localization of LKB1 by anchoring it in the cytoplasm. LYK5-LKB1 interaction results in phosphorylation of LYK5 and enhanced autophosphorylation of LKB1.

Gene ID: 92335

Pathways: AMPK Signaling