PHKB antibody (AA 45-160) (Cy7)

Catalog No. ABIN752824

This Rabbit Polyclonal antibody specifically detects PHKB in WB, IF (cc) and IF (p). It exhibits reactivity toward Human.

Quick Overview for PHKB antibody (AA 45-160) (Cy7) (ABIN752824)

Target: PHKB (Phosphorylase Kinase, beta (PHKB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHKB antibody is conjugated to Cy7

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-PHKB Antibody (Cy7)

Binding Specificity: AA 45-160

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PHKB

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PHKB

Target: PHKB (Phosphorylase Kinase, beta (PHKB))

Alternative Name: PHKB

Background:

Synonyms: Phosphorylase B, Phosphorylase kinase B, Phosphorylase kinase beta polypeptide, Phosphorylase kinase beta subunit, DKFZp781E15103, FLJ41698, KPBB_HUMAN.

Background: Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, encoded by this gene, which is a member of the phosphorylase b kinase regulatory subunit family. The gamma subunit also includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9B, also known as phosphorylase kinase deficiency of liver and muscle. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. Two pseudogenes have been found on chromosomes 14 and 20, respectively.[provided by RefSeq, Feb 2010].

Gene ID: 5257

Pathways: Cellular Glucan Metabolic Process