PDHA1 antibody (pSer293) (Cy5)

Catalog No. ABIN752882

The Rabbit Polyclonal anti-PDHA1 antibody has been validated for WB. It is suitable to detect PDHA1 in samples from Human, Mouse and Rat.

Quick Overview for PDHA1 antibody (pSer293) (Cy5) (ABIN752882)

Target: PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDHA1 antibody is conjugated to Cy5

Application: Western Blotting (WB)

Product Details anti-PDHA1 Antibody (Cy5)

Binding Specificity: pSer293

Specificity: This phosphorylation site is homologous across the listed species. This antibody will detect PDHA2, the testis specific form, when phosphorylated at Ser293 in Human, Ser296 in Mouse, and the predicted site of Ser296 in Rat due to sequence homology.

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human PDHA1 around the phosphorylation site of Ser293

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PDHA1

Target: PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))

Alternative Name: Pdha1

Background:

Synonyms: PDHA, PDHAD, PHE1A, PDHCE1A, Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHE1-A type I, PDHA1

Background: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

Gene ID: 5160

UniProt: P08559

Pathways: Warburg Effect