PKMYT1 antibody (AA 121-220)

Catalog No. ABIN754543

This Rabbit Polyclonal antibody specifically detects PKMYT1 in WB, ELISA, IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for PKMYT1 antibody (AA 121-220) (ABIN754543)

Target: PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKMYT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PKMYT1 Antibody

Binding Specificity: AA 121-220

Predicted Reactivity: Human,Mouse,Rat,Dog,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PKMYT1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PKMYT1

Target: PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))

Alternative Name: PKMYT1

Background:

Synonyms: cdc2 inhibitory kinase, Membrane associated tyrosine and threonine specic cdc2 inhibitory kinase, Membrane-associated tyrosine- and threonine-specic cdc2-inhibitory kinase, MYT1, Myt1 kinase, PKMYT 1, Pkmyt1, PMYT1_HUMAN, Protein kinase membrane associated tyrosine/threonine 1, Protein kinase Myt1, DKFZp547K1610, FLJ20093.

Background: PKMYT1 is a member of the serine/threonine protein kinase family. It preferentially phosphorylates and inactivates cell division cycle 2 protein (cdc2), and thus acts as a negative regulator of entry into mitosis (G2 to M transition). It mediates phosphorylation of cdc2 predominantly on 'Thr-14' and is also involved in Golgi fragmentation. It may be involved in phosphorylation of cdc2 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. It may be a downstream target of Notch signaling pathway during eye development. PKMYT1 is negatively regulated by hyperphosphorylation during mitosis.

Gene ID: 9088

Pathways: Mitotic G1-G1/S Phases, M Phase