ATF4 antibody (pSer245)

Catalog No. ABIN756172

This Rabbit Polyclonal antibody specifically detects ATF4 in WB, ELISA, IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for ATF4 antibody (pSer245) (ABIN756172)

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ATF4 Antibody

Binding Specificity: pSer245

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human ATF4 around the phosphorylation site of Ser245

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-ATF4 antibody (ABIN756172)

Jiang, Yun, Shi, Niu, Gao, Xie, Yu: "Downregulation of miR-384-5p attenuates rotenone-induced neurotoxicity in dopaminergic SH-SY5Y cells through inhibiting endoplasmic reticulum stress." in: American journal of physiology. Cell physiology, Vol. 310, Issue 9, pp. C755-63, (2016) (PubMed).

Hayashi, Ouchi, Endou, Anzai: "HOXB9 acts as a negative regulator of activated human T cells in response to amino acid deficiency." in: Immunology and cell biology, Vol. 94, Issue 6, pp. 612-7, (2016) (PubMed).

Target Details for ATF4

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Alternative Name: ATF4

Background:

Synonyms: ATF4 phospho S245, ATF4Ser245, Cyclic AMP response element binding protein 2, DNA binding protein TAXREB67, Activating Transcription Factor 4, ATF 4, ATF4 protein, CREB 2, CREB2, CREB-2, Cyclic AMP dependent transcription factor ATF 4, Tax Responsive Enhancer Element B67, TAXREB67, TXREB.

Background: ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain (referenced from Entrez gene).

Gene ID: 468

Pathways: Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response