SNRPE antibody (pSer446)

Catalog No. ABIN756787

This Rabbit Polyclonal antibody specifically detects SNRPE in WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It exhibits reactivity toward Mouse and Rat.

Quick Overview for SNRPE antibody (pSer446) (ABIN756787)

Target: SNRPE (Small Nuclear Ribonucleoprotein Polypeptide E (SNRPE))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNRPE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SNRPE Antibody

Binding Specificity: pSer446

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human B-Raf around the phosphorylation site of Ser446

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SNRPE

Target: SNRPE (Small Nuclear Ribonucleoprotein Polypeptide E (SNRPE))

Alternative Name: B-Raf

Background:

Synonyms: B Raf phospho S446, p-B Raf phospho S446, 94 kDa B raf protein, B raf 1, B Raf proto oncogene serine threonine protein kinase, BRAF 1, Braf, BRAF1, cRmil, MGC126806, MGC138284, Murine sarcoma viral v-raf oncogene homolog B1, Murine sarcoma viral v raf oncogene homolog B1, p94, RAFB 1, RAFB1, v raf murine sarcoma viral oncogene homolog B1, FLJ95109.

Background: The Raf kinases are important intermediates in signal transduction. Raf protein family members, including A Raf and B Raf, have intrinsic serine/threonine kinase activity. Interaction between Ras proteins and Raf proteins results in Raf-mediated phosphorylation and activation of MEK (also known as MAP kinase kinase). Defects in BRAF are involved in a wide range of cancers. B-Raf is a serine/threonine protein kinase that acts as a signal transducer from membrane-associated receptors to nuclear transcription factors. 1 BRAF is important for the regulation of cell proliferation and determination of cell fate during embryogenesis. BRAF acts downstream of Ras and upstream of MEK in the Ras-Raf-MEK-ERK signal transduction pathway, which is a conserved RAS-activated protein kinase cascade that regulates cell growth, proliferation, and differentiation in response to growth factors, cytokines, and hormones.

Gene ID: 673

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C