desert Hedgehog antibody (AA 101-200)

Catalog No. ABIN759686

The Rabbit Polyclonal anti-desert Hedgehog antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (fro) and IHC (p). It is suitable to detect desert Hedgehog in samples from Mouse.

Quick Overview for desert Hedgehog antibody (AA 101-200) (ABIN759686)

Target: desert Hedgehog (DHH) (Desert Hedgehog (DHH))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This desert Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-desert Hedgehog Antibody

Binding Specificity: AA 101-200

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DHH

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for desert Hedgehog

Target: desert Hedgehog (DHH) (Desert Hedgehog (DHH))

Alternative Name: DHH

Target Type: Species

Background:

Synonyms: Desert hedgehog Drosophila homolog, Desert hedgehog, Desert hedgehog homolog Drosophila, Desert hedgehog protein precursor.

Background: This gene encodes a member of the Hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved, the N terminal portion is soluble and contains the signalling activity while the C terminal portion is involved in precursor processing. More importantly, the C terminal product covalently attaches a cholesterol moiety to the N terminal product, restricting the N terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development.

Gene ID: 50846

Pathways: Hedgehog Signaling