Calreticulin antibody (AA 101-200)

Catalog No. ABIN759731

The Rabbit Polyclonal anti-Calreticulin antibody has been validated for WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect Calreticulin in samples from Human, Mouse and Rat. There are 3+ publications available.

Quick Overview for Calreticulin antibody (AA 101-200) (ABIN759731)

Target: Calreticulin (CALR)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calreticulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Calreticulin Antibody

Binding Specificity: AA 101-200

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Calreticulin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Calreticulin antibody (ABIN759731)

Tanaka, Kataoka, Yano, Sawada, Akashi, Inoue, Suzuki, Inagaki, Hayashi, Nishie, Shimura, Mizoshita, Mori, Kubota, Tanida, Takahashi, Joh: "Immunogenic cell death due to a new photodynamic therapy (PDT) with glycoconjugated chlorin (G-chlorin)." in: Oncotarget, Vol. 7, Issue 30, pp. 47242-47251, (2016) (PubMed).

Chen, Wu, Xu, Xu: "Calreticulin Binds to Fas Ligand and Inhibits Neuronal Cell Apoptosis Induced by Ischemia-Reperfusion Injury." in: BioMed research international, Vol. 2015, pp. 895284, (2015) (PubMed).

Angelova, Grekova, Heller, Kuhlmann, Soyka, Giese, Aprahamian, Bour, Rüffer, Cziepluch, Daeffler, Rommelaere, Werner, Raykov, Giese: "Complementary induction of immunogenic cell death by oncolytic parvovirus H-1PV and gemcitabine in pancreatic cancer." in: Journal of virology, Vol. 88, Issue 10, pp. 5263-76, (2014) (PubMed).

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: Calreticulin

Background:

Synonyms: RO, CRT, SSA, cC1qR, HEL-S-99n, Calreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 6, ERp6, HACBP, grp6, CALR, CRTC

Background: Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity).

Gene ID: 811

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response