SMYD3 antibody (AA 101-200) (Biotin)

Catalog No. ABIN760588

This Rabbit Polyclonal antibody specifically detects SMYD3 in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for SMYD3 antibody (AA 101-200) (Biotin) (ABIN760588)

Target: SMYD3 (SET and MYND Domain Containing 3 (SMYD3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMYD3 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SMYD3 Antibody (Biotin)

Binding Specificity: AA 101-200

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Cow,Sheep,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SMYD3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for SMYD3

Target: SMYD3 (SET and MYND Domain Containing 3 (SMYD3))

Alternative Name: SMYD3

Background:

Synonyms: SET and MYND domain containing 3, SET and MYND domain containing protein 3, SMYD 3, SMYD3 protein, Zinc finger MYND domain containing 1, Zinc finger MYND domain containing protein 1, Zinc finger protein subfamily 3A MYND domain containing 1, ZMYND 1, ZMYND1, ZNFN3A1, SMYD3_HUMAN.

Background: SMYD3 is a gene that is over-expressed in the majority of colorectal carcinomas and hepatocellular carcinomas. SMYD3 forms a complex with RNA polymerase II through an interaction with the RNA helicase HELZ and transactivates a set of genes that included oncogenes, homeobox genes and genes associated with cell-cycle regulation. SMYD3 binds to a motif, 5'-CCCTCC-3', present in the promoter region of downstream genes such as Nkx2.8. The SET domain of SMYD3 shows histone H3-lysine 4 (H3-K4)-specific methyltransferase activity, which is enhanced in the presence of the heat-shock protein HSP90A.

Gene ID: 64754