ASAH2 antibody (AA 701-780)

Catalog No. ABIN761276

The Rabbit Polyclonal anti-ASAH2 antibody has been validated for ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect ASAH2 in samples from Human.

Quick Overview for ASAH2 antibody (AA 701-780) (ABIN761276)

Target: ASAH2 (N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASAH2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ASAH2 Antibody

Binding Specificity: AA 701-780

Predicted Reactivity: Human,Mouse,Rat,Dog,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ASAH2

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ASAH2

Target: ASAH2 (N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2))

Alternative Name: ASAH2

Background:

Synonyms: Acylsphingosine deacylase 2, ASAH2, ASAH2_HUMAN, BCDase, hCD, HNAC1, LCDase, MGC129777, mitochondrial ceramidase, N acylsphingosine amidohydrolase non lysosomal ceramidase 2, N acylsphingosine amidohydrolase 2, N CDase, N-acylsphingosine amidohydrolase 2, N-CDase, NCDase, Neutral ceramidase soluble form, Non lysosomal ceramidase, Non-lysosomal ceramidase.

Background: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract.

Gene ID: 2298