SMARCB1 antibody (AA 301-385)

Catalog No. ABIN762566

The Rabbit Polyclonal anti-SMARCB1 antibody has been validated for ELISA, FACS, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect SMARCB1 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for SMARCB1 antibody (AA 301-385) (ABIN762566)

Target: SMARCB1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily B, Member 1 (SMARCB1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMARCB1 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SMARCB1 Antibody

Binding Specificity: AA 301-385

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SNF5

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SMARCB1 antibody (ABIN762566)

McAndrew, Gjidoda, Tagore, Miksanek, Floer: "Chromatin Remodeler Recruitment during Macrophage Differentiation Facilitates Transcription Factor Binding to Enhancers in Mature Cells." in: The Journal of biological chemistry, Vol. 291, Issue 35, pp. 18058-71, (2016) (PubMed).

Target Details for SMARCB1

Target: SMARCB1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily B, Member 1 (SMARCB1))

Alternative Name: Snf5

Background:

Synonyms: Snr1, TYE4, BAF47, hSNF5, INI1 antibody Integrase interactor 1 protein, RDT, Sfh1p, SMARCB1, SNF5, SNF5L1, Snr1, Sucrose nonfermenting yeast homolog like 1, SWI/SNF complex component SNF5, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1, SWI10, Transcription factor TYE4, Transcription regulatory protein SNF5.

Background: SNF5 is involved in transcriptional activation. The SWI/SNF complex is required for the induced expression of a large number of genes. This complex alters chromatin structure to facilitate binding of gene-specific dedicated transcription factors. SNF5 also funtions as a tumor suppressor gene and is frequently mutated in malignant rhabdoid tumors. It is also involved in adipocyte differentiation.

Gene ID: 6598

UniProt: Q12824