Human Adenovirus type 5 E1A (HAdV-5 E1A) (AA 181-280) antibody

Catalog No. ABIN762941

The Rabbit Polyclonal anti-Human Adenovirus type 5 E1A antibody is suitable to detect Human Adenovirus type 5 E1A in samples from Adeno-Associated Virus 5 (AAV-5), Rat and Virus. It has been validated for WB, IF (cc), IF (p), ELISA, IHC (fro) and IHC (p).

Quick Overview for Human Adenovirus type 5 E1A (HAdV-5 E1A) (AA 181-280) antibody (ABIN762941)

Target: Human Adenovirus type 5 E1A (HAdV-5 E1A)

Reactivity: Adeno-Associated Virus 5 (AAV-5), Rat, Virus

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 181-280

Cross-Reactivity: Rat, Virus

Cross-Reactivity (Details): Adenovirus5

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Adenovirus 5 E1A

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Human Adenovirus type 5 E1A (HAdV-5 E1A)

Target Type: Viral Protein

Background:

Synonyms: Adenovirus early E1A protein, Control protein E1A, E1A, Early E1A protein, Human adenovirus type 5, Human adenovirus C, pfam02703, E1A_ADE05.

Background: The early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11 % of the viral genome and consists of two transcriptional units E1A and E1B. E1A is sufficient for partial transformation and immortalization of primary cells. E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection and are able to either activate or repress the transcription of specific cellular genes. E1A forms specific complexes with cellular proteins including p105 causing inhibition of the cell cycle inducing arresting function of p105.

UniProt: P03255