KDM5B antibody (AA 65-165) (Cy5.5)

Catalog No. ABIN762991

This Rabbit Polyclonal antibody specifically detects KDM5B in WB, FACS, IF (cc) and IF (p). It exhibits reactivity toward Human and Rat.

Quick Overview for KDM5B antibody (AA 65-165) (Cy5.5) (ABIN762991)

Target: KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM5B antibody is conjugated to Cy5.5

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-KDM5B Antibody (Cy5.5)

Binding Specificity: AA 65-165

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Pig,Horse,Chicken,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human KDM5B/Jarid1B

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for KDM5B

Target: KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))

Alternative Name: KDM5B

Background:

Synonyms: CT31, PLU1, PUT1, PLU-1, JARID1B, PPP1R98, RBBP2H1A, Lysine-specific demethylase 5B, Cancer/testis antigen 31, Histone demethylase JARID1B, Jumonji/ARID domain-containing protein 1B, Retinoblastoma-binding protein 2 homolog 1, RBP2-H1, KDM5B, RBBP2H1

Background: Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma. Represses the CLOCK-ARNTL/BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity).

Gene ID: 10765

UniProt: Q9UGL1

Pathways: Warburg Effect