DNAJB9 antibody (AA 61-75)

Catalog No. ABIN768557

The Goat Polyclonal anti-DNAJB9 antibody is suitable to detect DNAJB9 in samples from Human and Mouse. It has been validated for WB, ELISA, IF and FACS.

Quick Overview for DNAJB9 antibody (AA 61-75) (ABIN768557)

Target: DNAJB9 (Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This DNAJB9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-DNAJB9 Antibody

Binding Specificity: AA 61-75

Purpose: DNAJB9 (aa61-75)

Sequence: PDAEAKFREI AEAYE

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PDAEAKFREIAEAYE, from the internal regoin of the protein sequence according to NP_036460.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 26-27 kDa band observed in Human Liver and Kidney lysates and approx.28 kDa in Mouse Liver lysates and in preliminary testing of Rat Liver lysate (calculated MW of 25.5 kDa according to Human NP_036460.1 and 25.6 kDa according to Mouse

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. R

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DNAJB9

Target: DNAJB9 (Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9))

Alternative Name: DNAJB9

Background: DNAJB9, DnaJ (Hsp40) homolog, subfamily B, member 9, DKFZp564F1862, ERdj4, MDG1, MST049, MSTP049, dnaJ homolog subfamily B member 9, endoplasmic reticulum DnaJ homolog 4, mdg-1, microvascular endothelial differentiation gene 1 protein

Molecular Weight: 25.5kDa according to NP_036460.1

Gene ID: 4189

NCBI Accession: NP_036460

Pathways: ER-Nucleus Signaling