IDH3B antibody (AA 33-46)

Catalog No. ABIN768560

The Goat Polyclonal anti-IDH3B antibody has been validated for WB and ELISA. It is suitable to detect IDH3B in samples from Human, Mouse, Rat and Pig.

Quick Overview for IDH3B antibody (AA 33-46) (ABIN768560)

Target: IDH3B (Isocitrate Dehydrogenase 3 (NAD+) beta (IDH3B))

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This IDH3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-IDH3B Antibody

Binding Specificity: AA 33-46

Purpose: IDH3B (aa33-46)

Sequence: HAASRSQAED VRVE

Specificity: This antibody is expected to recognize isoform a (NP_008830.2) and isoform b (NP_777280.1).

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HAASRSQAEDVRVE, from the internal region (near N Terminus) of the protein sequence according to NP_008830.2, NP_777280.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 39 kDa band observed in Mouse, Rat and Pig Heart lysates and in Human and Mouse Skeletal Muscle lysates (calculated MW of 41.9 kDa according to NP_777280.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IDH3B

Target: IDH3B (Isocitrate Dehydrogenase 3 (NAD+) beta (IDH3B))

Alternative Name: IDH3B

Background: IDH3B, isocitrate dehydrogenase 3 (NAD+) beta, FLJ11043, H-IDHB, MGC903, RP46, NAD(+)-specific ICDH subunit beta, NAD+-specific ICDH, NAD+-specific isocitrate dehydrogenase b subunit, NAD+-specific isocitrate dehydrogenase beta, isocitrate dehydrogenase [

Molecular Weight: 41.9kDa according to NP_777280.1

Gene ID: 3420, 170718, 94173

NCBI Accession: NP_008830, NP_777280