NOX1 antibody (Internal Region)

Catalog No. ABIN768583

This Goat Polyclonal antibody specifically detects NOX1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for NOX1 antibody (Internal Region) (ABIN768583)

Target: NOX1 (NADPH Oxidase 1 (NOX1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NOX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-NOX1 Antibody

Binding Specificity: Internal Region

Purpose: NOX1

Sequence: DKATDIVTGL KQK

Specificity: This antibody is expected to recognize both reported isoforms (NP_008983.2, NP_39249.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DKATDIVTGLKQK, from the internal region of the protein sequence according to NP_008983.2, NP_39249.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70-72 kDa band observed in lysates of cell lines A549, CaCo-2, HeLa and U937, (calculated MW of 64.9 kDa according to NP_008983.2,). This molecular weight is routinely observed by other sources and was successfully blocked by incubatio

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NOX1

Target: NOX1 (NADPH Oxidase 1 (NOX1))

Alternative Name: NOX1

Background: NOX1, NADPH oxidase 1, GP91-2, MOX1, NOH-1, NOH1, MOX-1, NADH/NADPH mitogenic oxidase subunit P65-MOX, NADPH oxidase 1 variant NOH-1L, NADPH oxidase homolog-1, NOX-1, mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating), mitogenic oxida

Molecular Weight: 64.9kDa according to NP_008983.2

Gene ID: 27035, 237038

NCBI Accession: NP_008983, NP_39249

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Proton Transport