NRF2 antibody (AA 445-458)

Catalog No. ABIN768601

This Goat Polyclonal antibody specifically detects NRF2 in WB, ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for NRF2 antibody (AA 445-458) (ABIN768601)

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NRF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-NRF2 Antibody

Binding Specificity: AA 445-458

Purpose: NRF2 (aa445-458)

Sequence: KHSSRLEAHL TRDE

Specificity: This antibody is expected to recognize all reported isoforms (

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KHSSRLEAHLTRDE, from the internal region of the protein sequence according to NP_006155.2, NP_001138884.1, NP_001138885.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in Human Lung lysates and approx 75 kDa in preliminary testing of Mouse and Rat Liver and KNRK, MDCK, and nuclear NIH3T3 cell lysate (calculated MW of 67.8 kDa according to NP_006155.2). This molecular weight rate

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended conc

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NRF2

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Alternative Name: NFE2L2

Background: NFE2L2, nuclear factor (erythroid-derived 2)-like 2, NRF2, HEBP1, NF-E2-related factor 2, NFE2-related factor 2, OTTHUMP00000205251, OTTHUMP00000207980, nuclear factor erythroid 2-related factor 2, nuclear factor, erythroid derived 2, like 2

Molecular Weight: 67.8kDa according to NP_006155.2

Gene ID: 4780, 18024, 83619

NCBI Accession: NP_006155, NP_001138884, NP_001138885

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling