SON antibody (N-Term)

Catalog No. ABIN768615

This anti-SON antibody is a Goat Polyclonal antibody detecting SON in ELISA and IHC. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for SON antibody (N-Term) (ABIN768615)

Target: SON (SON DNA Binding Protein (SON))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SON antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-SON Antibody

Binding Specificity: N-Term

Purpose: SON

Sequence: KSRCVSVQTD PTD

Specificity: This antibody is expected to recognize reported isoforms B ( NP_115571.1) and F (NP_620305.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KSRCVSVQTDPTD, from the N Terminus of the protein sequence according to NP_115571.1, NP_620305.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 5 μg/mL.

Western Blot: Preliminary experiments gave an approx 35 kDa band in Human, Mouse and Rat Heart lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculate

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-SON antibody (ABIN768615)

Lu, Ang, Zhuang: "Spatially resolved epigenomic profiling of single cells in complex tissues." in: Cell, Vol. 185, Issue 23, pp. 4448-4464.e17, (2022) (PubMed).

Target Details for SON

Target: SON (SON DNA Binding Protein (SON))

Alternative Name: SON

Background: SON, SON DNA binding protein, BASS1, C21orf50, DBP-5, FLJ21099, FLJ33914, KIAA1019, NREBP, SON3, Bax antagonist selected in Saccharomyces 1, NRE-binding protein, OTTHUMP00000197908, OTTHUMP00000198934, negative regulatory element-binding protein, protein

Molecular Weight: 35kDa

Gene ID: 6651, 20658, 304092

NCBI Accession: NP_115571, NP_620305