TGFBR1 antibody (AA 50-63)

Catalog No. ABIN768623

The Goat Polyclonal anti-TGFBR1 antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect TGFBR1 in samples from Human and Rat.

Quick Overview for TGFBR1 antibody (AA 50-63) (ABIN768623)

Target: TGFBR1 (Transforming Growth Factor, beta Receptor 1 (TGFBR1))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This TGFBR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-TGFBR1 Antibody

Binding Specificity: AA 50-63

Purpose: TGFBR1 (aa50-63)

Sequence: TDGLCFVSVT ETTD

Specificity: This antibody is expected to recognize both reported isoforms (NP_004603.1, NP_001124388.1). The immunizing peptide represents part of a potential extracellular domain.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence TDGLCFVSVTETTD, from the internal region of the protein sequence according to NP_004603.1, NP_001124388.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Adrenal Gland and Small Intestine. Recommended concentration: 5 μg/mL.

Western Blot: Approx 55 kDa band observed in Rat Liver and Rat Lung lysates (calculated MW of 55.6 kDa according to Rat NP_036907.2). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Immunofluorescence: This antibody is not recommended for IF testing.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TGFBR1

Target: TGFBR1 (Transforming Growth Factor, beta Receptor 1 (TGFBR1))

Alternative Name: TGFBR1

Background: TGFBR1, transforming growth factor, beta receptor 1, AAT5, ACVRLK4, ALK-5, ALK5, LDS1A, LDS2A, SKR4, TGFR-1, TGF-beta receptor type I, TGF-beta receptor type-1, TGF-beta type I receptor, activin A receptor type II-like kinase, 53kD, activin A receptor typ

Molecular Weight: 55.6kDa according to Rat NP_036907.2

Gene ID: 7046, 21812, 29591

NCBI Accession: NP_004603, NP_001124388

Pathways: Growth Factor Binding