Caspase 3 antibody (Internal Region)

Catalog No. ABIN768625

This Goat Polyclonal antibody specifically detects Caspase 3 in WB, ELISA, ICC and IF. It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for Caspase 3 antibody (Internal Region) (ABIN768625)

Target: Caspase 3 (CASP3)

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-Caspase 3 Antibody

Binding Specificity: Internal Region

Purpose: Caspase 3

Sequence: RDVSKEDHSK RS

Specificity: This antibody is expected to recognise both reported isoforms (NP_004337.2 and NP_116786.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RDVSKEDHSKRS, from the internal region of the protein sequence according to NP_004337.2, NP_116786.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 32 kDa band observed in lysates of cell line MOLT4 and in Mouse Liver lysates (calculated MW of 31.6 kDa according to Human NP_004337.2 and 31.4 kDa according to Mouse NP_033940.1, NP_001271338.1). An additional band of 65 kDa was also

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10μg/ml. This antibody has been successfully used in ICC on Rat: Hóngyi Zhào et al. (2018) PMID: 29853981.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Caspase 3 antibody (ABIN768625)

Zhào, Liu, Zeng, Li, Zhang, Huang: "Troxerutin and Cerebroprotein Hydrolysate Injection Protects Neurovascular Units from Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury In Vitro." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 9859672, (2018) (PubMed).

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: CASP3

Background: CASP3, caspase 3, caspase 3, apoptosis-related cysteine protease, HGNC:1504, caspase 3, apoptosis-related cysteine peptidase, CPP32, CPP32B, SCA-1, PARP cleavage protease, SREBP cleavage activity 1, Yama, apopain, cysteine protease CPP32, procaspase3

Molecular Weight: 31.6kDa according to NP_004337.2

Gene ID: 836

NCBI Accession: NP_004337, NP_116786

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation