DAPP1 antibody (Internal Region)

Catalog No. ABIN768627

This anti-DAPP1 antibody is a Goat Polyclonal antibody detecting DAPP1 in WB and ELISA. Suitable for Human.

Quick Overview for DAPP1 antibody (Internal Region) (ABIN768627)

Target: DAPP1 (Dual Adaptor of Phosphotyrosine and 3-phosphoinositides (DAPP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DAPP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-DAPP1 Antibody

Binding Specificity: Internal Region

Purpose: DAPP1 / BAM32

Sequence: KLSQIRKQLN QGE

Cross-Reactivity: Cow, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KLSQIRKQLNQGE, from the internal region (near C Terminus) of the protein sequence according to NP_055210.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: In transfected HEK293 transiently expressing full-length Human DAPP1 (myc and DYKDDDDK tagged), a band of approx. 35 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag a

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DAPP1

Target: DAPP1 (Dual Adaptor of Phosphotyrosine and 3-phosphoinositides (DAPP1))

Alternative Name: DAPP1

Background: DAPP1, dual adaptor of phosphotyrosine and 3-phosphoinositides, BAM32, DKFZp667E0716

Molecular Weight: 35kDa

Gene ID: 27071

NCBI Accession: NP_055210

Pathways: BCR Signaling