GAD antibody (Internal Region)

Catalog No. ABIN768644

The Goat Polyclonal anti-GAD antibody has been validated for WB and ELISA. It is suitable to detect GAD in samples from Mouse.

Quick Overview for GAD antibody (Internal Region) (ABIN768644)

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This GAD antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-GAD Antibody

Binding Specificity: Internal Region

Purpose: GAD25 / GAD67

Sequence: QRTNSLEEKS R

Specificity: This antibody is expected to recognize both reported isoforms (NP_038473.2, NP_000808.2).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QRTNSLEEKSR, from the internal region of the protein sequence according to NP_038473.2, NP_000808.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in Mouse Brain lysates (calculated MW of 66.6 kDa according to Mouse NP_032103.2). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GAD

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Alternative Name: GAD1

Background: GAD1, glutamate decarboxylase 1 (brain, 67 kDa), FLJ45882, GAD, SCP, 67 kDa glutamic acid decarboxylase, GAD-67, OTTHUMP00000204990, OTTHUMP00000204993, OTTHUMP00000204994, OTTHUMP00000204995, glutamate decarboxylase 1, glutamate decarboxylase 67 kDa isofo

Molecular Weight: 66.6kDa according to Mouse NP_032103.2

Gene ID: 2571

NCBI Accession: NP_038473, NP_000808