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D Amino Acid Oxidase antibody (AA 286-298)

The Goat Polyclonal anti-D Amino Acid Oxidase antibody has been validated for WB and ELISA. It is suitable to detect D Amino Acid Oxidase in samples from Human. There is 1 publication available.
Catalog No. ABIN768647

Quick Overview for D Amino Acid Oxidase antibody (AA 286-298) (ABIN768647)

Target

See all D Amino Acid Oxidase (DAO) Antibodies
D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Reactivity

  • 37
  • 10
  • 6
  • 5
Human

Host

  • 39
  • 4
  • 3
  • 2
Goat

Clonality

  • 45
  • 3
Polyclonal

Conjugate

  • 30
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This D Amino Acid Oxidase antibody is un-conjugated

Application

  • 34
  • 30
  • 11
  • 9
  • 9
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA

Grade

Verified
  • Binding Specificity

    • 10
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    AA 286-298

    Purpose

    D-amino-acid oxidase (aa286-298)

    Sequence

    RPQIRLEREQ LRT

    Cross-Reactivity

    Cow, Dog, Human, Pig

    Purification

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Immunogen

    Peptide with sequence C-RPQIRLEREQLRT, from the internal region of the protein sequence according to NP_001908.3.

    Isotype

    IgG
  • Application Notes

    Western Blot: Approx 37 kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 39.5 kDa according to NP_001908.3). Recommended concentration: 0.03-0.01 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Minimize freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Sasabe, Miyoshi, Rakoff-Nahoum, Zhang, Mita, Davis, Hamase, Waldor: "Interplay between microbial d-amino acids and host d-amino acid oxidase modifies murine mucosal defence and gut microbiota." in: Nature microbiology, Vol. 1, Issue 10, pp. 16125, (2018) (PubMed).

  • Target

    D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

    Alternative Name

    DAO

    Background

    DAO, D-amino-acid oxidase, DAAO, DAMOX, MGC35381, OXDA

    Molecular Weight

    39.5kDa according to NP_001908.3

    Gene ID

    1610

    NCBI Accession

    NP_001908
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