APOL1 antibody (AA 297-309)

Catalog No. ABIN768657

This anti-APOL1 antibody is a Goat Polyclonal antibody detecting APOL1 in WB and ELISA. Suitable for Human.

Quick Overview for APOL1 antibody (AA 297-309) (ABIN768657)

Target: APOL1 (Apolipoprotein L, 1 (APOL1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-APOL1 Antibody

Binding Specificity: AA 297-309

Purpose: APOL1 (aa297-309)

Sequence: PHASASRPRV TEP

Specificity: This antibody is expected to recognize all three reported isoforms (NP_003652.2, NP_663318.1, NP_001130013.1). Reported variants represent identical protein: NP_003652.2, NP_001130012.1

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PHASASRPRVTEP, from the internal region of the protein sequence according to NP_003652.2, NP_663318.1, NP_001130013.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 42+45 kDa bands observed in Human Placenta lysates (calculated MW of 45.9 kDa and 42.2 kDa according to NP_663318.1 and NP_001130013.1 resp.). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for APOL1

Target: APOL1 (Apolipoprotein L, 1 (APOL1))

Alternative Name: APOL1

Background: APOL1, apolipoprotein L, 1, APO-L, APOL, APOL-I, OTTHUMP00000197984, OTTHUMP00000197985, OTTHUMP00000197986, OTTHUMP00000197987, OTTHUMP00000198062, apolipoprotein L-I, apolipoprotein L1

Molecular Weight: 45.9kDa and 42.2kDa according to NP_663318.1 and NP_001130013.1 resp.

Gene ID: 8542

NCBI Accession: NP_003652, NP_663318, NP_001130013