CD83 antibody (FITC)

Catalog No. ABIN775716

The FITC-conjugated Mouse Monoclonal anti-CD83 antibody (Clone HB15e) (ABIN775716) specifically detects CD83 in FACS. The antibody is reactive with Human and Non-Human Primate samples.

Quick Overview for CD83 antibody (FITC) (ABIN775716)

Target: CD83

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD83 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: HB15e

Product Details anti-CD83 Antibody (FITC)

Purpose: Anti-Hu CD83 FITC

Cross-Reactivity (Details): Human, Non-Human Primates

Characteristics: The mouse monoclonal antibody HB15e recognizes an extracellular epitope of CD83, a 40-45 kDa type I glycoprotein expressed on mature dendritic cells.

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human CD83-transfected Cos cells

Isotype: IgG1, kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze,Protect from prolonged exposure to light

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for CD83

Target: CD83

Alternative Name: CD83

Background: CD83 Molecule,CD83 is a 40-45 kDa heavily glycosylated type I cell surface glycoprotein of immunoglobulin family. It is expressed on the surface of mature dendritic cells, Langerhans cells in the skin, and interdigitating reticulum cells in the lymphoid tissues. Low expression of CD83 has been reported in activated T and B cells. Cytoplasmic expression of CD83 can be detected also in monocytes and macrophages. CD83 is involved in modulation of antigen presentation. Soluble CD83 has immunoregulatory functions, it is able to down-regulate dendritic cell maturation and stimulation of T cells. In the developing immune system, release of soluble CD83 from dendritic cells upon stimulation by gram-positive or gram-negative bacteria has anti-allergic effect. Herpes simplex virus, on the other hand, causes CD83 degradation in mature dendritic cells.,BL11, HB15

Gene ID: 9308

UniProt: Q01151