C3 antibody (Biotin)

Catalog No. ABIN783938

The Goat Polyclonal anti-C3 antibody has been validated for WB, IP, IHC (fro), EIA, IF and ID. It is suitable to detect C3 in samples from Mouse.

Quick Overview for C3 antibody (Biotin) (ABIN783938)

Target: C3 (Complement Component 3 (C3))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This C3 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunodiffusion (ID)

Product Details anti-C3 Antibody (Biotin)

Characteristics: Molar Ratio: Biotin/IgG ~6.2

Purification: The IgG fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion.

Immunogen: C3c isolated and purified from pooled normal Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1 mL of sterile distilled water.

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid Repeated thawing and freezing.

Target Details for C3

Target: C3 (Complement Component 3 (C3))

Alternative Name: Complement C3

Background: C3 is the most abundant complement protein in rat serum. Its biological function strongly resembles that of C3 in man and other laboratory animal species. It has a central role in the activation system being common to both pathways. Activation of C3 is achieved by very specific limited proteolysis resulting in the release of a number of degradation fragments. The anaphylotoxin C3a promotes smooth muscle contraction and increases vascular permeability: the large C3b fragment is involved in binding to the complement activator and can be interact with specific receptors to allow efficient clearance of the activating cell or particle, degradation fragments of C3b (C3bi, C3c, C3dg C3d) are important in receptor binding and clearance mechanisms, in virus neutralization and possibly in the immune response.Synonyms: CPAMD1, Complement component 3

Gene ID: 10116

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling