ENOS antibody

Catalog No. ABIN784153

This Rabbit Polyclonal antibody specifically detects ENOS in WB. It exhibits reactivity toward Human, Rat, Mouse, Pig, Cow, Sheep, Monkey and Horse.

Quick Overview for ENOS antibody (ABIN784153)

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reactivity: Human, Rat, Mouse, Pig, Cow, Sheep, Monkey, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENOS antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ENOS Antibody

Specificity: The antibody recognizes 140 kDa eNOS.

Cross-Reactivity (Details): Species reactivity (expected):Pig, Horse, Sheep, Cattle, Monkey.
Species reactivity (tested):Human, Mouse, and Rat.

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide surrounding amino acid 602 of Rat eNOS

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 30 % Glycerol, 0.5 % BSA, and 0.01 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. For long term storage aliquot and freeze at -70 °C.

Target Details for ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Alternative Name: NOS3

Background: ENOS (Endothelial nitric-oxide synthase) is an important enzyme in the cardiovascular system. eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495. Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli. In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation.Synonyms: Constitutive NOS, EC-NOS, Endothelial NOS, NOS type III, Nitric oxide synthase, eNOS, endothelial

Gene ID: 4846

NCBI Accession: NP_000594

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling