Doublecortin antibody (AA 151-161)

Catalog No. ABIN793226

The Goat Polyclonal anti-Doublecortin antibody is suitable to detect Doublecortin in samples from Human, Mouse, Rat, Cow, Pig, Horse, Dog, Monkey, Rabbit, Bat and Hamster. It has been validated for WB and ELISA.

Quick Overview for Doublecortin antibody (AA 151-161) (ABIN793226)

Target: Doublecortin (DCX)

Reactivity: Human, Mouse, Rat, Cow, Pig, Horse, Dog, Monkey, Rabbit, Bat, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This Doublecortin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Doublecortin Antibody

Binding Specificity: AA 151-161

Specificity: Human DCX. This antibody is expected to recognize all four reported isoforms (NP_000546.2, NP_835365.1, NP_835364.1, NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Cat, Horse, Rabbit, Pig (100%) Turkey, Chicken (91%) Lizard (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KTSANMKAPQS from an internal region of human DCX (NP_000546.2, NP_835365.1, NP_835364.1, NP_001182482.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Cat, Horse, Rabbit, Pig (100%), Turkey, Chicken (91%), Lizard (82%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:32000), WB (0.01 - 0.03 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 45kD band observed in Mouse fetal Brain lysates (calculated MW of 49.3kD according to Human NP_000546.2 and 40.6kD according to Human NP_835365.1 and Mouse NP_001103692.1). Recommended concentration: 0.01-0.03 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Doublecortin

Target: Doublecortin (DCX)

Alternative Name: DCX / Doublecortin

Background: Name/Gene ID: DCX

Synonyms: DCX, DBCN, Doublecortin, Doublin, Doublecortex, Lis-X, SCLH, LISX, XLIS, Lissencephalin-X

Gene ID: 1641

NCBI Accession: NP_000546, NP_835365, NP_835364, NP_001182482

UniProt: O43602