Doublecortin antibody (AA 151-161)

Catalog No. ABIN793226

Product Details anti-Doublecortin Antibody

Target: Doublecortin (DCX)

Binding Specificity: AA 151-161

Reactivity: Human, Mouse, Rat, Cow, Pig, Horse, Dog, Monkey, Rabbit, Bat, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This Doublecortin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human DCX. This antibody is expected to recognize all four reported isoforms (NP_000546.2, NP_835365.1, NP_835364.1, NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Cat, Horse, Rabbit, Pig (100%) Turkey, Chicken (91%) Lizard (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KTSANMKAPQS from an internal region of human DCX (NP_000546.2, NP_835365.1, NP_835364.1, NP_001182482.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Cat, Horse, Rabbit, Pig (100%), Turkey, Chicken (91%), Lizard (82%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:32000), WB (0.01 - 0.03 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 45kD band observed in Mouse fetal Brain lysates (calculated MW of 49.3kD according to Human NP_000546.2 and 40.6kD according to Human NP_835365.1 and Mouse NP_001103692.1). Recommended concentration: 0.01-0.03 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Doublecortin

Target: Doublecortin (DCX)

Alternative Name: DCX / Doublecortin (DCX Products)

Background: Name/Gene ID: DCX

Synonyms: DCX, DBCN, Doublecortin, Doublin, Doublecortex, Lis-X, SCLH, LISX, XLIS, Lissencephalin-X

Gene ID: 1641

NCBI Accession: NP_000546, NP_835365, NP_835364, NP_001182482

UniProt: O43602