HSF1 antibody (pSer303)
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- Target See all HSF1 Antibodies
- HSF1 (Heat Shock Factor Protein 1 (HSF1))
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Binding Specificity
- pSer303
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This HSF1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity
- Human
- Predicted Reactivity
- Mouse,Rat,Dog,Pig,Rabbit
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic phosphopeptide derived from human HSF1 around the phosphorylation site of Ser303
- Isotype
- IgG
- Top Product
- Discover our top product HSF1 Primary Antibody
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anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Alkaline Phosphatase (AP))
HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 Alkaline Phosphatase (AP)
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (HRP)HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 HRP
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (FITC)HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 FITC
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (APC)HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 APC
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Biotin)HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 Biotin
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PE)HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 PE
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PerCP)HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 PerCP
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 488)HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 Atto 488
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 594)HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 Atto 594
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 390)HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 Atto 390
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- Application Notes
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- HSF1 (Heat Shock Factor Protein 1 (HSF1))
- Alternative Name
- HSF1 (HSF1 Products)
- Background
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Synonyms: HSF1Ser303, Heat shock factor 1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1, hsf1, HSTF 1, HSTF1.
Background: Two HSFs have been identified in human cells, HSF 1 and HSF 2, which bind to the same HSEs and have 38 % sequence identity. These factors are activated by distinct stimuli, HSF 1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF 2 is activated during hemin-mediated differentiation of human erythroleukemia cells.HSF 1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF 1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF 1 is necessary for maximal transcription of heat shock genes.
- Gene ID
- 3297
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