HSP70 antibody (pTyr525)

Catalog No. ABIN800988

This anti-HSP70 antibody is a Rabbit Polyclonal antibody detecting HSP70 in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). Suitable for Human and Mouse.

Quick Overview for HSP70 antibody (pTyr525) (ABIN800988)

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP70 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HSP70 Antibody

Binding Specificity: pTyr525

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Dog,Cow,Sheep,Pig,Chicken,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human HSP70 around the phosphorylation site of Tyr525

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: HSP70

Background:

Synonyms: HSP70 phospho Tyr525, HSP70 phospho Y525, p-HSP70 Tyr525, p-HSP70 Y525, HSP70, HSP-70, HSP 70, Heat shock 70 kDa protein 1, heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1B, Heat shock induced protein, heat shock protein 70, HSP70 1, HSP70 2, HSP70.1, HSP72, HSPA1, HSPA1A, HSPA1B, XXbac BCX40G17.3 001, Heat shock 70 kDa protein 1A/1B, HSP71_HUMAN, Hspa1a, Hsp70-1, HSP72, Hspa1, Hspa1b, Heat shock 70 kDa protein 1A/1B, shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP70.1/HSP70.2.

Background: This intronless gene encodes a 70 kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008].

Gene ID: 3303, 3304