GIT1 antibody (pTyr545) (Cy3)

Catalog No. ABIN801111

This anti-GIT1 antibody is a Rabbit Polyclonal antibody detecting GIT1 in WB, IF (cc) and IF (p). Suitable for Human.

Quick Overview for GIT1 antibody (pTyr545) (Cy3) (ABIN801111)

Target: GIT1

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GIT1 antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-GIT1 Antibody (Cy3)

Binding Specificity: pTyr545

Predicted Reactivity: Human,Mouse,Rat,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human GIT1 around the phosphorylation site of Tyr545

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GIT1

Target: GIT1

Alternative Name: GIT1

Background:

Synonyms: GIT1 phospho Tyr545, p-GIT1 Tyr545, ARF GAP GIT1, ARF GTPase activating protein GIT1, CAT 1, Cat1, Cool associated and tyrosine phosphorylated protein 1, G protein coupled receptor kinase interactor 1, GRK interacting protein 1.

Background: Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist (1,2). G protein-coupled receptor kinases (GRKs) are activated by activated G protein-coupled receptors, and they function to phosphorylate and inactivate cell surface receptors in the heterotrimeric G protein signaling cascade (3,4). GIT1 (for GRK-interactor 1) and GIT2 are GTPase-activating proteins (GAP) for members of the ADP ribosylation factor (ARF) family of small GTP-binding proteins, which are involved in vesicular trafficking (5,6). GIT1 overexpression results in reduced internalization and resensitization of b2-adrenergic receptor, thus reducing b2-adrenergic receptor signaling (5).

Gene ID: 28964