IL1R1 antibody (pTyr496)

Catalog No. ABIN801423

Product Details anti-IL1R1 Antibody

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Binding Specificity: pTyr496

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1R1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human IL-1R1 around the phosphorylation site of Tyr496

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for IL1R1

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Alternative Name: IL-1R1 (IL1R1 Products)

Background:

Synonyms: P8, IL1R, IL1RA, CD121A, D2S1473, IL-1R-alpha, Interleukin-1 receptor type 1, IL-1R-1, IL-1RT-1, IL-1RT1, CD121 antigen-like family member A, Interleukin-1 receptor alpha, Interleukin-1 receptor type I, IL1R1, IL1RT1

Background: Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex.

Gene ID: 3554

UniProt: P14778

Pathways: NF-kappaB Signaling, Carbohydrate Homeostasis, Cancer Immune Checkpoints